光散射/荧光比率法测定全氟辛烷磺酸

在pH=1.81的Britton Robinson（BR）缓冲溶液中，全氟辛烷磺酸（PFOS）与罗丹明B（RhB）通过静电引力和疏水作用使RhB的光散射增强，荧光发生猝灭。 利用在普通荧光光度计上单次扫描获得的340 nm处的光散射强度（I340）与596 nm处的荧光强度（F596）之比，建立了测定PFOS的光散射/荧光比率法，检出限（3σ）为17 nmol/L。 方法用于环境水样中PFOS的测定，RSD≤4.2%。

Determination of Perfluorooctane Sulfonate by Light Scattering-Fluorescence Ratio Method

Perfluorooctane sulfonate(PFOS) can quench the fluorescence of rhodamine B(RhB) in pH=1.81 Britton-Robinson(BR) buffer solution, and results in light scattering enhancement by electrostatic forces and hydrophobic interactions. In this work, a light scattering-fluorescence ratio method of PFOS was established with a common spectrofluorometer by measuring the light emission signals through one scanning, out of which a ratio of the intensity of light scattering at 340 nm to that of fluorescence at 596 nm could be obtained. The linear range is from 0.17 to 10.0 μmol/L, and the limit of detection(3σ) is 17 nmol/L. The method has been applied to the determination of PFOS in the environmental samples with RSD≤4.2%.