Observation and analysis of single DNA nano-kinetics by pin-fiber video scope |
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| Authors: | Hirakawa Yasuyuki Suzuto Masashi Ohnishi Hitoshi Shingaki Tomoteru Eyring Edward M Tokunaga Makio Masujima Tsutomu |
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| Institution: | Graduate School of Medical Science, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. yhirakaw@hiroshima-u.ac.jp |
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| Abstract: | The nano-kinetic movement of a single DNA molecule was observed and analyzed by a newly developed video-microscope system with an optical fiber, called a pin-fiber video scope. A single lambda-DNA molecule was put in focus using fiber-illumination, and the stretching and shrinking motion was measured. The molecule's kinetics were analyzed by numerical calculations and are discussed. A photocleavage phenomenon of DNA molecules was also visualized by the pin-fiber video scope. The new video-microscope system has the potential to observe and analyze the nano-kinetics of a single molecule. |
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