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Synthesis and surface properties of new semi-fluorinated sulfobetaines potentially usable for 2D-electrophoresis |
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| Authors: | Pascal Thebault Mireille Starita-Geribaldi Serge Geribaldi |
| Institution: | a Laboratoire de Chimie des Matériaux Organiques et Métalliques, Institut de Chimie, Université de Nice Sophia-Antipolis, Parc Valrose, 06108 Nice Cedex 2, France b Connexines et Proliferation Germinale, Physiopathologie Cellulaire et Moléculaire (INSERM U670), UFR de Medecine, Université de Nice Sophia-Antipolis, 28 Avenue de Valombrose, 06107 Nice Cedex 1, France |
| Abstract: | New semi-fluorinated amidosulfobetaines, homologs of hydrocarbon amidosulfobetaines (ASB) commonly used in two-dimensional gel electrophoresis (2DE), were prepared in three steps from 2-F-alkylethyl iodide or F-alkyl iodide. Their synthesis was described and their air-water interface properties were investigated and compared with their perhydrogenated counterpart properties. The influence of the relative lengths of the perfluorinated and hydocarbonated moieties was discussed. 2DE of a rat testicular membrane fraction was performed comparatively using one of these fluorinated sulfobetaines and its hydrocarbon homolog; these preliminary results showed the great potential of the semi-fluorinated sulfobetaines in proteomic analysis. |
| Keywords: | Sulfobetaine Semi-fluorinated Surfactant Critical micelle concentration Surface tension 2D electrophoresis |
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