Automated image-based method for laboratory screening of coating libraries for adhesion of algae and bacterial biofilms |
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Authors: | Ribeiro Eraldo Stafslien Shane J Cassé Franck Callow James A Callow Maureen E Pieper Robert J Daniels Justin W Bahr James A Webster Dean C |
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Institution: | Computer Sciences, Florida Institute of Technology, Melbourne, Florida 32901, USA. |
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Abstract: | Assessment and down-selection of non-biocidal coatings that prevent the adhesion of fouling organisms in the marine environment requires a hierarchy of laboratory methods to reduce the number of experimental coatings for field testing. Automated image-based methods are described that facilitate rapid, quantitative biological screening of coatings generated through combinatorial polymer chemistry. Algorithms are described that measure the coverage of bacterial and algal biofilms on coatings prepared in 24-well plates and on array panels, respectively. The data are used to calculate adhesion strength of organisms on experimental coatings. The results complement a number of physical and mechanical methods developed to screen large numbers of samples. |
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