Intensified biochip system using chemiluminescence for the detection of Bacillus globigii spores |
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Authors: | Dimitra N Stratis-Cullum Guy D Griffin Joel Mobley Tuan Vo-Dinh |
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Institution: | (1) US Army Research Laboratory, AMSRD-ARL-SE-EO, 2800 Powder Mill Road, Adelphi, MD 20783, USA;(2) Fitzpatrick Institute for Photonics, Departments of Biomedical Engineering and Chemistry, Duke University, 2589 Fitzpatrick Center, Durham, NC 27708, USA;(3) Department of Physics and Astronomy, The University of Mississippi, 1025 NCPA, Oxford, MS 38677, USA |
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Abstract: | This paper reports the first intensified biochip system for chemiluminescence detection and the feasibility of using this
system for the analysis of biological warfare agents is demonstrated. An enzyme-linked immunosorbent assay targeting Bacillus globigii spores, a surrogate species for Bacillus anthracis, using a chemiluminescent alkaline phosphatase substrate is combined with a compact intensified biochip detection system.
The enzymatic amplification was found to be an attractive method for detection of low spore concentrations when combined with
the intensified biochip device. This system was capable of detecting approximately 1 × 105
Bacillus globigii spores. Moreover, the chemiluminescence method, combined with the self-contained biochip design, allows for a simple, compact
system that does not require laser excitation and is readily adaptable to field use.
Figure Schematic diagram of the miniature biochip detection system |
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Keywords: | Biochips Biosensors immunoassays ELISA Spectroscopy Bacillus globigii Chemiluminescence Spores Intensifier Bioluminescence |
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