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Intensified biochip system using chemiluminescence for the detection of Bacillus globigii spores
Authors:Dimitra N Stratis-Cullum  Guy D Griffin  Joel Mobley  Tuan Vo-Dinh
Institution:(1) US Army Research Laboratory, AMSRD-ARL-SE-EO, 2800 Powder Mill Road, Adelphi, MD 20783, USA;(2) Fitzpatrick Institute for Photonics, Departments of Biomedical Engineering and Chemistry, Duke University, 2589 Fitzpatrick Center, Durham, NC 27708, USA;(3) Department of Physics and Astronomy, The University of Mississippi, 1025 NCPA, Oxford, MS 38677, USA
Abstract:This paper reports the first intensified biochip system for chemiluminescence detection and the feasibility of using this system for the analysis of biological warfare agents is demonstrated. An enzyme-linked immunosorbent assay targeting Bacillus globigii spores, a surrogate species for Bacillus anthracis, using a chemiluminescent alkaline phosphatase substrate is combined with a compact intensified biochip detection system. The enzymatic amplification was found to be an attractive method for detection of low spore concentrations when combined with the intensified biochip device. This system was capable of detecting approximately 1 × 105 Bacillus globigii spores. Moreover, the chemiluminescence method, combined with the self-contained biochip design, allows for a simple, compact system that does not require laser excitation and is readily adaptable to field use. MediaObjects/216_2008_1835_Figa_HTML.gif Figure Schematic diagram of the miniature biochip detection system
Keywords:Biochips  Biosensors immunoassays  ELISA  Spectroscopy            Bacillus globigii            Chemiluminescence  Spores  Intensifier  Bioluminescence
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