| pH值敏感相分离特性的蛋白质酶解试剂研究 |
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| 引用本文： | 吴蓓蓓,商云岭,邬建敏.pH值敏感相分离特性的蛋白质酶解试剂研究[J].分析化学,2011,39(1):12-16. |
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| 作者姓名： | 吴蓓蓓 商云岭 邬建敏 |
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| 作者单位： | 浙江大学化学系微分析系统研究所; |
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| 基金项目： | 国家转基因重大专项(No.2009ZX08011-014B)资助 |
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| 摘 要： | 通过DEC/NHS偶联反应将胰蛋白酶(Trypsin)连接到具有pH值敏感性的壳聚糖(CS)链上.Trypsin-CS偶联物保留了CS的pH值敏感相分离特性及Trypsjn的酶催化活性.浊度滴定实验表明,此偶联物的临界相分离pH值(CPSP)仍保持在偏中性范围内,并且随CS的分子量降低而略有上升.这种偏中性的CPSP值...
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| 关 键 词： | pH值敏感高分子 胰蛋白酶 偶联 酶活性 肽谱分析 |
pH Sensitive Phase Transition and Enzyme Activity of Trypsin-Chitosan Conjugant |
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| WU Bei-Bei,SHANG Yun-Ling,WU Jian-Min.pH Sensitive Phase Transition and Enzyme Activity of Trypsin-Chitosan Conjugant[J].Chinese Journal of Analytical Chemistry,2011,39(1):12-16. |
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| Authors: | WU Bei-Bei SHANG Yun-Ling WU Jian-Min |
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| Institution: | WU Bei-Bei,SHANG Yun-Ling,WU Jian-Min*(Institute of Microanalytical System,Department of Chemistry,Zhejiang University,Hangzhou 310027) |
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| Abstract: | Trypsin-chitosan conjugant was prepared by coupling trypsin with chitosan(CS) through the method of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide/N-hudroxysuccinamide(EDC/NHS) coupling reactions.The resulting conjugant had borne both characteristics of pH-sensitive phase transitional behavior and enzyme catalytic activity.The critical phase transitional pH point(CPSP) of the conjugant was determined by turbid titration method.The results show that CPSP value increase with the decrease of chitosan molecular ... |
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| Keywords: | pH sensitive polymers Trypsin Conjugation Enzyme activity peptide fragment mapping |
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