| 槲寄生药材的高效液相色谱指纹图谱研究 |
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| 引用本文： | 袁庆芳,赵春梅,张忠鹏,杨官娥.槲寄生药材的高效液相色谱指纹图谱研究[J].分析科学学报,2011,27(1):16-20. |
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| 作者姓名： | 袁庆芳 赵春梅 张忠鹏 杨官娥 |
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| 作者单位： | 山西医科大学药学院中药学教研室;东北林业大学帽儿山教学区; |
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| 基金项目： | 国家自然科学基金(No.30672621); 山西省自然科学基金(No.2006011199); 山西医科大学博士启动基金(03200811) |
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| 摘 要： | 采用Diamonsil C<,18>(2)色谱柱,乙腈-0.1%磷酸梯度洗脱,测定了15批不同产地的槲寄生样品,建立其指纹图谱共有模式;采用"中药色谱指纹图谱相似度评价系统"软件进行数据处理,聚类分析,建立槲寄生药材高效液相色谱指纹图谱.结果表明:以高圣草素-7-O-β-D-芹菜糖基(1→2)-β-D-葡萄糖苷为参照物...
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| 关 键 词： | 槲寄生 指纹图谱 反相高效液相色谱法 |
Investigation on the High Performance Liquid Chromatography Fingerprints of Viscum coloratum(Komar)Nakai |
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| YUAN Qing-fang,ZHAO Chun-mei,ZHANG Zhong-peng,YANG Guan-e.Investigation on the High Performance Liquid Chromatography Fingerprints of Viscum coloratum(Komar)Nakai[J].Journal of Analytical Science,2011,27(1):16-20. |
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| Authors: | YUAN Qing-fang ZHAO Chun-mei ZHANG Zhong-peng YANG Guan-e |
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| Institution: | YUAN Qing-fang1,ZHAO Chun-mei2,ZHANG Zhong-peng1,YANG Guan-e1(1.Department of TCM,Shanxi Medical University,Taiyuan 030001,2.Teaching Zone of MaoEr mountain,Northeast Forestry University,Haerbin 150611) |
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| Abstract: | Reversed phase-high performance liquid chromatography(RP-HPLC) was used to investigate the fingerprints of Viscum coloratum.15 batches of Viscum coloratum samples from different areas were analyzed by RP-HPLC with acetonitrile-phosphoric acid solution(0.1%) as the mobile phase and gradient elution on a Diamonsil C18(2) column was processed.Similarity Evaluation System for Chromatographic Fingerprint of TCM and clustering method were used for data analysis and fingerprint investigation.18 chromatographic pea... |
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| Keywords: | Viscum coloratum(Komar) Nakai Fingerprint chromatogram Reversed phase-high performance liquid chromatography |
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