| 管式磁性微粒子化学发光免疫分析法测定人尿液中的雌三醇 |
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| 引用本文： | 郑国金,方卢秋,陈惠,李振甲,应希堂,林金明.管式磁性微粒子化学发光免疫分析法测定人尿液中的雌三醇[J].分析化学,2011,39(1):62-66. |
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| 作者姓名： | 郑国金 方卢秋 陈惠 李振甲 应希堂 林金明 |
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| 作者单位： | 楚雄医药高等专科学校化学教研室;清华大学化学系;长江师范学院化学化工学院;北京科美东雅生物技术有限公司; |
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| 基金项目： | 国家自然科学基金(No.90813015)资助项目 |
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| 摘 要： | 待测尿液中的雌三醇、辣根过氧化物酶标记的雌三醇与异硫氰酸荧光素(FITC)标记的兔抗雌三醇抗体在均相体系中发生竞争性免疫反应,再加入用羊抗FITC抗体包被的磁微粒,反应生成物结合在磁微粒上,在磁场中经分离、洗涤后加发光底物,检测发光强度(RLU),测定尿液中雌三醇的含量。通过对检测条件的优化,建立了磁性微粒子化学发光免疫分析法测定人尿液中雌三醇的方法,并对正常男性、女性和孕妇的尿液中雌三醇含量进行了测定。结果表明,本方法的线性范围为1～100μg/L,检出限为0.25μg/L,具有很高的灵敏度;批内相对标准偏差<14%;批间相对标准偏差<7%,具有良好的稳定性和重现性。
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| 关 键 词： | 雌三醇 化学发光免疫分析 磁性微粒子 尿液 |
Magnetic Microparticle Chemiluminescence Immunoassay Method for Determination of Estrioi in Human Urine |
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| ZHENG Guo-Jin,FANG Lu-Qiu,CHEN Hui,LI Zhen-Jia,YING Xi-Tang,LIN Jin-Ming.Magnetic Microparticle Chemiluminescence Immunoassay Method for Determination of Estrioi in Human Urine[J].Chinese Journal of Analytical Chemistry,2011,39(1):62-66. |
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| Authors: | ZHENG Guo-Jin FANG Lu-Qiu CHEN Hui LI Zhen-Jia YING Xi-Tang LIN Jin-Ming |
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| Institution: | ZHENG Guo-Jin1,4,FANG Lu-Qiu2,CHEN Hui4,LI Zhen-Jia3,YING Xi-Tang3,LIN Jin-Ming* 4 1(Department of Chemistry,Chuxiong Medical College,Chuxiong 675000) 2(Department of Chemical Engineering and Yangtze Normal University,Chongqing 408100) 3(Beijin Chemclin Biotech Co.,Ltd,Beijing 100012) 4(Department of Chemistry,Tsinghua University,Beijing100084) |
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| Abstract: | A magnetic microparticle chemiluminescence immunoassay method was developed for the determination of estriol in human urine.The sample of estriol and horseradish peroxidase(HRP) labeled estriol with fluorescein isothiocyanate(FITC) labeled rabbit anti-estriol antibodies were mixed in homogeneous system.The reaction product in homogeneous system can be combined onto the goat anti-FITC antibody-coated magnetic particles.After the magnetic field separation of bond and free,the light-emitting substrate was adde... |
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| Keywords: | Estriol Chemiluminescence immunoassay Magnetic particles Urine |
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