| 新型纳米纤维固相萃取柱制备及其用于组织样品中柔红霉素测定研究 |
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| 引用本文： | 曲子健,康学军,吴玮玮,陈宝安,顾忠泽. 新型纳米纤维固相萃取柱制备及其用于组织样品中柔红霉素测定研究[J]. 分析化学, 2011, 39(3): 312-316. DOI: 10.3724/SP.J.1096.2011.00312 |
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| 作者姓名： | 曲子健 康学军 吴玮玮 陈宝安 顾忠泽 |
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| 作者单位： | 东南大学苏州研究院苏州环境与生物安全重点实验室;东南大学公共卫生学院;东南大学儿童发展与学习科学教育部重点实验室;东南大学中大医院血液科; |
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| 基金项目： | 国家“973”基金项目资助(No.2007CB936300); 国家自然基金项目资助(No.30872970); 苏州市应用基础研究项目资助(Nos.SYJG0912,SYN201006); 江苏省工业支撑项目(No.BE2010088)资助 |
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| 摘 要： | 研制出一种新型纳米纤维固相萃取柱,并将其用于大鼠心、肝、脾、肺、肾组织及血浆中柔红霉素的净化、浓缩处理,建立了生物样品中柔红霉素的高效液相荧光检测法.以10%(V/V)HClO4为溶剂,对组织样进行匀浆和离心处理,上清液用纳米纤维小柱净化富集,以50 μL含1%(V/V)冰醋酸的甲醇洗脱,进样20μL检测.流动相为甲醇...
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| 关 键 词： | 柔红霉素 纳米纤维 高效液相色谱 |
Preparation of Novel Packed-Nanofibers Solid Phase Extraction Column and Its Application to the Determination of Daunorubicin in Rat Tissues |
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| QU Zi-Jian,KANG Xue-Jun,WU Wei-Wei,CHEN Bao-An,GU Zhong-Ze. Preparation of Novel Packed-Nanofibers Solid Phase Extraction Column and Its Application to the Determination of Daunorubicin in Rat Tissues[J]. Chinese Journal of Analytical Chemistry, 2011, 39(3): 312-316. DOI: 10.3724/SP.J.1096.2011.00312 |
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| Authors: | QU Zi-Jian KANG Xue-Jun WU Wei-Wei CHEN Bao-An GU Zhong-Ze |
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| Affiliation: | QU Zi-Jian1,KANG Xue-Jun1,2,WU Wei-Wei3,CHEN Bao-An3,GU Zhong-Ze11(Suzhou Key Laboratory of Enviroment and Biosafety,School of Public Health,Southeast University,Suzhou 215123)2(Key Laboratory of Child Development and Learning Science(Ministry of Education),Research Center for Learning Science,Nanjing 210096)3(Department of Hematology,Zhongda Hospital,Clinical Medical College,Nanjing 210009) |
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| Abstract: | A novel packed-nanofibers solid-phase extraction column has been investigated for purification and enrichment of daunorubicin in plasma and different tissues of rat(heart,liver,spleen,lung,kidney).Meanwhile,an HPLC method with fluorescence detection for determination of biological samples has been deve-loped.10%(V/V) HClO4 was used to extract daunorubicin from biological samples.After homogenizing for 1min and centrifuging for 10 min at 12000 r/min,the supernatant was purified and preconcentrated by the col... |
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| Keywords: | Daunorubicin Nanofiber High performance liquid chromatography |
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