| 恒电位自动滴定法分析脂肪酶水解活性影响因素 |
| |
| 引用本文： | 赵炜,黄卓楠,李娜. 恒电位自动滴定法分析脂肪酶水解活性影响因素[J]. 分析化学, 2011, 39(3): 351-355. DOI: 10.3724/SP.J.1096.2011.00351 |
| |
| 作者姓名： | 赵炜 黄卓楠 李娜 |
| |
| 作者单位： | 西安交通大学能动学院化工系; |
| |
| 基金项目： | 国家重点基础研究计划(973项目)项目(No.2009CB2199006); 国家自然科学基金项目(No.20406014)资助 |
| |
| 摘 要： | 利用恒电位自动滴定法分析了脂肪酶(Candida rugosa lipase,CRL)催化橄榄油水解活性的影响因素,确定了适宜的酶活性测定条件,对比了传统表面活性剂和咪唑类离子液体对酶活性的影响.37 ℃时,与酶蛋白浓度呈线性关系的酶活性测定范围为1～14 μmol/min;酶蛋白浓度范围为0.01～0.07 g/L;...
|
| 关 键 词： | 恒电位滴定 假丝酵母脂肪酶 酶活性 离子液体 表面活性剂 |
Factors Affecting Lipase Activity Assay by Automatic pH-Stat Potentiometric Titration Method |
| |
| ZHAO Wei,HUANG Zhuo-Nan,LI Na. Factors Affecting Lipase Activity Assay by Automatic pH-Stat Potentiometric Titration Method[J]. Chinese Journal of Analytical Chemistry, 2011, 39(3): 351-355. DOI: 10.3724/SP.J.1096.2011.00351 |
| |
| Authors: | ZHAO Wei HUANG Zhuo-Nan LI Na |
| |
| Affiliation: | (Department of Chemical Engineering,Xi′an Jiao Tong University,Xi′an,710049) |
| |
| Abstract: | An accurate quantitative analytical method of lipase activity was established.The activity of Candida rugosa lipase(CRL) in the hydrolysis reaction of olive oil was analyzed by automatic pH-stat potentiometric titration method.Various factors influencing lipase activity assay were discussed and appropriate analysis conditions were selected.The results showed that the hydrolytic activity of CRL was linearly proportional to enzyme protein concentration only within 0.01-0.07 g/L range of protein concentration ... |
| |
| Keywords: | Automatic pH-stat potentiometric titration method Candida rugosa lipase Lipase activity Ionic liquid Surfactant |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
