| 在线超声提取96-孔盘技术高通量快速测定全叶马兰中果聚糖成分 |
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| 引用本文： | 田尚衣,万泓志,黄国辉,杨允菲,张义飞,邵帅.在线超声提取96-孔盘技术高通量快速测定全叶马兰中果聚糖成分[J].分析化学,2011,39(3):397-400. |
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| 作者姓名： | 田尚衣 万泓志 黄国辉 杨允菲 张义飞 邵帅 |
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| 作者单位： | 东北师范大学草地科学研究所植被生态科学教育部重点实验室;东北师范大学生命科学学院生物基础实验教学中心; |
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| 基金项目： | 国家自然科学基金项目(No.30770397); 东北师范大学生物学基地项目(No.J0830627)资助 |
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| 摘 要： | 采用在线动态超声提取装置对全叶马兰果聚糖成分进行提取,用效应面方法优化了在线提取条件.提取溶剂为纯水,待测样品为5 mg,提取条件为超声功率是496 W,溶剂流速 0.63 Ml/min,溶剂体积 4.69 Ml;用96-孔盘联合酚硫酸程序对果聚糖进行高通量快速检测,果聚糖的加标回收率为98.3%～105.7%,相对标...
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| 关 键 词： | 全叶马兰 果聚糖 高通量分析 在线动态超声提取 96-孔盘 苯酚 |
Dynamic Ultrasound-assisted Extraction and High-throughput for Analysis of Fructans from Kalimeris Integrifolia by 96-Well Format |
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| TIAN Shang-Yi,WAN Hong-Zhi,HUANG Guo-Hui,YANG Yun-Fei,ZHANG Yi-Fei,SHAO Shuai.Dynamic Ultrasound-assisted Extraction and High-throughput for Analysis of Fructans from Kalimeris Integrifolia by 96-Well Format[J].Chinese Journal of Analytical Chemistry,2011,39(3):397-400. |
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| Authors: | TIAN Shang-Yi WAN Hong-Zhi HUANG Guo-Hui YANG Yun-Fei ZHANG Yi-Fei SHAO Shuai |
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| Institution: | TIAN Shang-Yi1,2,WAN Hong-Zhi1,HUANG Guo-Hui2,YANG Yun-Fei1,ZHANG Yi-Fei1,SHAO Shuai11(Institute of Grassland Science,Northeast Normal University,Key Laboratory of Vegetation Ecology,Ministry of Education,Changchun 130024)2(Central Laboratory of General Biology,School of Life Sciences,Changchun 130024) |
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| Abstract: | An on-line method was developed for the extraction and determination of fructans in plant samples.Fructans was extracted with water by ultrasound assisted,and directly introduced into a well of 96-well format which was reacted with concentrated sulphuric acid and 5% phenol for fast quantifying fructans.Response surface methodology(RSM) along with Box-Behnken design(BBD) was applied to optimize the critical variables of the extraction conditions of dynamic ultrasound-assisted extraction(DUAE) system for fruc... |
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| Keywords: | Kalimeris integrifolia Fructans High-throughput analysis Dynamic ultrasoun-dassisted extraction 96-Well format Phenol |
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