| 酶联免疫吸附分析法检测花生过敏原的研究 |
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| 引用本文： | 邵景东,孙秀兰,张银志,王毅谦,宁炜,杨振东,周伟杰. 酶联免疫吸附分析法检测花生过敏原的研究[J]. 分析科学学报, 2011, 27(1): 89-92 |
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| 作者姓名： | 邵景东 孙秀兰 张银志 王毅谦 宁炜 杨振东 周伟杰 |
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| 作者单位： | 张家港出入境检验检疫局;江南大学食品学院;江南大学食品科学与技术国家重点实验室;无锡市疾病预防控制中心; |
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| 基金项目： | 国家自然科学基金(No.20806033); “十一五”国家科技支撑计划(No.2007BAK36B06); 江南大学食品科学与技术国家重点实验室自由探索项目(No.SKLF-20092060204); 张家港市科技局项目(No.ZKS0808) |
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| 摘 要： | 本文利用自己研制的花生过敏原免疫新西兰大耳兔,获得效价为200 000的抗花生过敏原特异性抗体,建立了花生过敏原蛋白的间接竞争酶联免疫(ELISA)检测方法.结果表明:花生抗原在0.01～100 ng/mL范围内具有较好的线性关系,其竞争标准曲线为v=-0.1212x+0.9285(r=0.9819),IC50为34....
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| 关 键 词： | 花生 过敏原 酶联免疫 检测 |
An Enzyme-linked Immunosorbent Assay Method for Detection of Allergens in Peanut |
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| SHAO Jing-dong,SUN Xiu-lan,ZHANG Yin-zhi,WANG Yi-qian,NING Wei,YANG Zhen-dong,ZHOU Wei-jie. An Enzyme-linked Immunosorbent Assay Method for Detection of Allergens in Peanut[J]. Journal of Analytical Science, 2011, 27(1): 89-92 |
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| Authors: | SHAO Jing-dong SUN Xiu-lan ZHANG Yin-zhi WANG Yi-qian NING Wei YANG Zhen-dong ZHOU Wei-jie |
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| Affiliation: | SHAO Jing-dong1,SUN Xiu-lan2,ZHANG Yin-zhi2,WANG Yi-qian1,NING Wei2,YANG Zhen-dong2,ZHOU Wei-jie4(1.Zhangjiagang Entry-exit inspection And Quarantine,Zhangjiagang 215500,2.The State Key Lab of Food Science and Technology,Jiangnan University,Wuxi 214122,3.School of Food Science & Technology,Wuxi 214036,4.Wuxi Center for Disease Control and Prevention,Wuxi 214023) |
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| Abstract: | Peanut allergen was used to immune New Zealand big-eared rabbit to harvest antibody of 2000 000 valence,and indirect competitive enzyme-linked immunosorbent assay(ELISA) method was used in the allergens detection.The results show that there was a good linearity between 0.01 ng/mL and 100 ng/mL.The Ic50 was 34.28 ng/mL and detection limit was 0.1 ng/mL. |
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| Keywords: | Peanut Allergens Enzyme-linked immunosorbent assay Detection |
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