Selective recruitment of membrane protein complexes onto gold substrates patterned by dip-pen nanolithography |
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Authors: | Valiokas Ramūnas Vaitekonis Sarūnas Klenkar Goran Trinkūnas Gediminas Liedberg Bo |
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Affiliation: | Division of Molecular Physics, Department of Physics, Chemistry and Biology, Link?ping University, S-581 83 Link?ping, Sweden. valiokas@ar.fi.lt |
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Abstract: | Dip-pen nanolithography (DPN) is employed to develop a generic array platform for the selective recruitment of membrane protein complexes. An atomic force microscope tip inked with HS(CH2)16NH2 is used to generate amino-terminated domains on gold. These domains can be arranged into microscopic and submicroscopic patterns, and the untreated gold substrate is subsequently blocked with HS(CH2)2CONH(CH2CH2O)15CH3, a compound known to resist the unspecific binding of proteins and cells. The patterned gold substrate is exposed to an enriched membrane fraction from mutant Rhodobacter sphaeroides, which contains photosynthetic core complexes consisting of the reaction center and the light-harvesting complex LH1. The selective recruitment to the patterned domains, governed primarily by electrostatic interactions, is confirmed by contact mode atomic force microscopy. |
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