Analysis of aqueous 2,4,6-trinitrotoluene (TNT) using a fluorescent displacement immunoassay |
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| Authors: | Goldman Ellen R Anderson George P Lebedev Nikolai Lingerfelt Brian M Winter Phan T Patterson Charles H Mauro J Matthew |
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| Institution: | US Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, DC 20375, Washington, USA. erg@cbmse.nrl.navy.mil |
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| Abstract: | We report a rapid, simple, and sensitive assay that is potentially amenable to high throughput screening for analysis of 2,4,6-trinitrotoluene (TNT) present in aqueous solutions. The assay is based on the change in fluorescence emission intensity of a fluorescently labeled TNT analogue pre-bound to an anti-TNT antibody that occurs upon its competitive displacement by TNT. The assay can be performed in both cuvette- and 96-well plate-based formats. TNT at a level of 0.5 micro g L(-1) (0.5 ppb) was detected in phosphate buffered saline; detection improved to 0.05 micro g L(-1) (0.05 ppb) for TNT dissolved in artificial seawater. |
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