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| 反相高效液相色谱法测定不同烟叶中的游离氨基酸 | |
| 引用本文： | 陈文,王存文,王光辉,金亚利,李丹,陈祖刚,马舒翼.反相高效液相色谱法测定不同烟叶中的游离氨基酸[J].化学分析计量,2007,16(1):14-16. |
| 作者姓名： | 陈文 王存文 王光辉 金亚利 李丹 陈祖刚 马舒翼 |
| 作者单位： | 1. 武汉工程大学化工与制药学院,武汉,430074 2. 武汉科技大学化工学院,武汉,430081 3. 武汉烟草集团技术中心,武汉,430050 |
| 摘 要： | 用乙醇水溶液提取烟叶中的游离氨基酸并通过阳离子交换柱纯化后,采用OPA(邻苯二甲醛丹酰氯)、FMOC(9-芴基甲氧基羰酰氯)联合在线衍生反相高效液相色谱法对烤烟、白肋烟和香料烟中的游离氨基酸进行了对比研究,发现白肋烟、香料烟和烤烟的烟叶中所含游离氨基酸总量分别为14.0mg/g、7.7mg/g和3.3mg/g;三者在所含主要氨基酸种类上存在显著差别。用该方法考察了不同产地烟叶中游离氨基酸的含量。 |
| 关 键 词： | 高效液相色谱法 烟叶 游离氨基酸 烤烟 白肋烟 香料烟 |
| 修稿时间： | 2006年9月18日 |
DETERMINATION OF FREE AMINO ACIDS IN DIFFERENT TOBACCOS BY REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
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| Chen Wen,Wang Cunwen,Wang Guanghui,Jin Yali,Li Dan,Chen Zugang,Ma Shuyi.DETERMINATION OF FREE AMINO ACIDS IN DIFFERENT TOBACCOS BY REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J].Chemical Analysis And Meterage,2007,16(1):14-16. | |
| Authors: | Chen Wen Wang Cunwen Wang Guanghui Jin Yali Li Dan Chen Zugang Ma Shuyi |
| Abstract: | Free amino acids in tobacco were extracted with 80% (mass percent) ethanol liquor and then were purified through cation-exchange column. After extraction and purification, reversed phase high performance liquid chromatography with precolumn derivatization with OPA(Dansyl-Cl)and FMOC(fluoryl-chlorocarbonate)were employed to determine free amino acids in burley tobacco, oriental tobacco and flue-cured tobacco. The results showed that the total amount of free amino acids in burley tobacco was the largest, 14.0 mg/g, and that of oriental tobacco was the second, approximately 7.79 mg/g, and the flue-cured tobacco was lowest, about 3.30 mg/g. The main free amino acids in the three tobaccos were different. The method was used to determine the free amino asids in tobacco from different producing area. |
| Keywords: | high performance liquid chromatography tobacco free amino acid burley tobacco oriental tobacco flue-cured tobacco |
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