| 钯配合物[Pd(phen)(L-asp)]·3H2O的结构，DNA键合和细胞活性研究 |
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| 作者姓名： | GAO Enjun LIU Lei SHI Chunyue YIN Hongxi ZHU Mingchang WU Qiong LIU Qitao |
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| 作者单位： | a Laboratory of Coordination Chemistry, Shenyang Institute of Chemical Technology, Shenyang, ;Liaoning 110142, China ;b School of Chemistry, Northeast Normal University, Changchun, Jilin 130024, China ;c College of Chemistry, Liaoning University, Shenyang, Liaoning 110036, China ; |
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| 摘 要： | 本文用常温方法合成了钯配合物，化学式Pd(phen)(L-asp)]•3H2O，并用元素分析和红外图谱的方法进行分析，经单晶X射线衍射对其结构表征。以顺铂为参照，研究了该配合物对三种不同癌细胞（人宫颈癌细胞，肝癌细胞，口腔癌细胞）的细胞毒素作用，结果证明该钯配合物对人宫颈癌细胞有较强的抑制作用。通过多种光谱手段同时研究了该配合物与鱼精DNA作用模式，结果说明通过插入方式阻断鱼精DNA的复制。同时，测定配合物与pBR322质粒DNA作用的凝胶电泳。
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| 关 键 词： | Pd配合物 细胞活性 DNA键合 |
| 收稿时间： | 2008-9-15 |
| 修稿时间： | 2008-10-17 |
Structure,DNA Binding Studies and Cytotoxicity of Complex [Pd(phen)(L‐asp)]·3H2O |
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| GAO Enjun,LIU Lei,SHI Chunyue,YIN Hongxi,ZHU Mingchang,WU Qiong,LIU Qitao.Structure,DNA Binding Studies and Cytotoxicity of Complex [Pd(phen)(L‐asp)]·3H2O[J].Chinese Journal of Chemistry,2009,27(6):1061-1066. |
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| Authors: | Enjun GAO Lei LIU Chunyue SHI Hongxi YIN Mingchang ZHU Qiong WU Qitao LIU |
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| Institution: | 1. Tel.: 0086‐24‐89385016;2. Fax: 0086‐24‐89388211;3. Laboratory of Coordination Chemistry, Shenyang Institute of Chemical Technology, Shenyang, Liaoning 110142, China;4. School of Chemistry, Northeast Normal University, Changchun, Jilin 130024, China;5. College of Chemistry, Liaoning University, Shenyang, Liaoning 110036, China |
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| Abstract: | The palladium(II) complex of Pd(phen)(L‐asp)]·3H2O (phen=1,10‐phenanthroline, H2L‐asp=L‐aspartic acid) has been synthesized from a solution reaction and analyzed by elemental analyses, 1H NMR and IR spectra. Moreover, the complex has been structurally characterized by single‐crystal X‐ray diffractometry. The cytotoxicity assay of the complex and cis‐DDP as reference substance against three different cancer cell lines (Hela, Hep‐G2 and KB) has been conducted. The results show that the Pd complex exhibits higher cytotoxicity against Hela system. The study on the interaction of the Pd complex with fish sperm DNA (FS‐DNA) has been performed with diverse spectroscopic techniques, showing that the complex is bound to the fish sperm DNA via an intercalative mode. Gel electrophoresis assay demonstrates the ability of the complex to cleave the pBR 322 plasmid DNA. |
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| Keywords: | palladium complex cytotoxic activity NA‐binding |
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