Gold nanoparticle-enhanced target (AuNPET) as universal solution for laser desorption/ionization mass spectrometry analysis and imaging of low molecular weight compounds |
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Authors: | Justyna Sekuła Joanna Nizioł Wojciech Rode Tomasz Ruman |
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Affiliation: | 1. Rzeszów University of Technology, Faculty of Chemistry, Bioorganic Chemistry Laboratory, 6 Powstańców Warszawy Ave., 35-959 Rzeszów, Poland;2. Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland |
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Abstract: | Preparation is described of a durable surface of cationic gold nanoparticles (AuNPs), covering commercial and custom-made MALDI targets, along with characterization of the nanoparticle surface properties and examples of the use in MS analyses and MS imaging (IMS) of low molecular weight (LMW) organic compounds. Tested compounds include nucleosides, saccharides, amino acids, glycosides, and nucleic bases for MS measurements, as well as over one hundred endogenous compounds in imaging experiment. The nanoparticles covering target plate were enriched in sodium in order to promote sodium-adduct formation. The new surface allows fast analysis, high sensitivity of detection and high mass determination accuracy. Example of application of new Au nanoparticle-enhanced target for fast and simple MS imaging of a fingerprint is also presented. |
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Keywords: | Gold nanoparticles Low molecular weight compounds MALDI Mass spectrometry Matrix Matrix-assisted laser desorption/ionization Matrix-free LDI Matrixless LDI Nanoparticles SALDI |
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