Rapid Legionella pneumophila determination based on a disposable core–shell Fe3O4@poly(dopamine) magnetic nanoparticles immunoplatform |
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| Authors: | Miriam Martín Pedro Salazar Carmen Jiménez María Lecuona Mª José Ramos Jesús Ode Julia Alcoba Rossany Roche Reynaldo Villalonga Susana Campuzano José Manuel Pingarrón José Luis González-Mora |
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| Institution: | 1. Neurochemistry and Neuroimaging Group, Laboratory of Sensors, Biosensors and Materials, Faculty of Medical Sciences, University of La Laguna, Campus de Ofra s/n, 38071, La Laguna, Tenerife, Spain;2. Atlántica Biomédica S.L., Tenerife, Spain;3. Informática y Equipamiento Médico de Canarias S.A., Tenerife, Spain;4. Laboratory of Nanotechnology on Surfaces, Institute of Materials Science of Seville (CSIC-Univ. Sevilla), Calle Américo Vespucio 49, 41092, Sevilla, Spain;5. Department of Microbiology and Infection Control, Hospital Universitario de Canarias, Tenerife, Spain;6. Unit Service of Microbiology, Hospital Universitario Nuestra Señora de La Candelaria, Tenerife, Spain;g Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain |
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| Abstract: | A novel amperometric magnetoimmunoassay, based on the use of core–shell magnetic nanoparticles and screen-printed carbon electrodes, was developed for the selective determination of Legionella pneumophila SG1. A specific capture antibody (Ab) was linked to the poly(dopamine)–modified magnetic nanoparticles (MNPs@pDA-Ab) and incubated with bacteria. The captured bacteria were sandwiched using the antibody labeled with horseradish peroxidase (Ab-HRP), and the resulting MNPs@pDA-Ab-Legionella neumophila-Ab-HRP were captured by a magnetic field on the electrode surface. The amperometric response measured at −0.15 V vs. Ag pseudo-reference electrode of the SPCE after the addition of H2O2 in the presence of hydroquinone (HQ) was used as transduction signal. The achieved limit of detection, without pre-concentration or pre-enrichment steps, was 104 Colony Forming Units (CFUs) mL−1. The method showed a good selectivity and the MNPs@pDA-Ab exhibited a good stability during 30 days. The possibility of detecting L. pneumophila at 10 CFU mL−1 level in less than 3 h, after performing a membrane-based preconcentration step, was also demonstrated. |
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| Keywords: | Legionella pneumophila Magnetic nanoparticles poly(dopamine) Electrochemical immunoassay |
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