Label-free electrochemical aptasensor constructed by layer-by-layer technology for sensitive and selective detection of cancer cells |
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Authors: | Tianshu Wang Jiyang Liu Xiaoxiao Gu Dan Li Jin Wang Erkang Wang |
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Affiliation: | 1. College of Physics, Jilin University, Changchun, Jilin 130012, China;2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China;3. Department of Chemistry, Physics and Applied Mathematics, State University of New York at Stony Brook, Stony Brook, NY 11794-3400, USA |
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Abstract: | Here, a cytosensor was constructed with ferrocene-appended poly(allylamine hydrochloride) (Fc-PAH) functionalized graphene (Fc-PAH-G), poly(sodium-p-styrenesulfonate) (PSS) and aptamer (AS1411) by layer-by-layer assembly technology. The hybrid nanocomposite Fc-PAH-G not only brings probes on the electrode and also promotes electron transfer between the probes and the substrate electrode. Meanwhile, LBL technology provides more effective probes to enhance amplified signal for improving the sensitivity of the detection. While AS1411 forming G-quardruplex structure and binding cancer cells, the current response of the sensing electrode decreased due to the insulating properties of cellular membrane. Differential pulse voltammetry (DPV) was performed to investigate the electrochemical detection of HeLa cells attributing to its sensitivity of the current signal change. The as-prepared aptasensor showed a high sensitivity and good stability, a widely detection range from 10 to 106 cells/mL with a detection limit as low as 10 cells/mL for the detection of cancer cells. |
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Keywords: | Graphene-based nanocomposite Electrochemical aptasensor Layer-by-layer technology Differential pulse voltammetry Cancer cells |
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