Hydrazine selective dual signaling chemodosimetric probe in physiological conditions and its application in live cells |
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Authors: | Sandip Nandi Animesh Sahana Sandip Mandal Archya Sengupta Ansuman Chatterjee Damir A. Safin Maria G. Babashkina Nikolay A. Tumanov Yaroslav Filinchuk Debasis Das |
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Affiliation: | 1. Department of Chemistry, The University of Burdwan, Burdwan, 713104 West Bengal, India;2. Department of Zoology, Visva Bharati University, Santiniketan, West Bengal, India;3. Institute of Condensed Matter and Nanosciences, Molecules, Solids and Reactivity (IMCN/MOST), Université catholique de Louvain, Place L. Pasteur 1, 1348 Louvain-la-Neuve, Belgium |
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Abstract: | A rhodamine–cyanobenzene conjugate, (E)-4-((2-(3′,6′-bis(diethylamino)-3-oxospiro[isoindoline-1,9′-xanthene]-2-yl)ethylimino)methyl)benzonitrile (1), which structure has been elucidated by single crystal X-ray diffraction, was synthesized for selective fluorescent “turn-on” and colorimetric recognition of hydrazine at physiological pH 7.4. It was established that 1 detects hydrazine up to 58 nM. The probe is useful for the detection of intracellular hydrazine in the human breast cancer cells MCF-7 using a fluorescence microscope. Spirolactam ring opening of 1, followed by its hydrolysis, was established as a probable mechanism for the selective sensing of hydrazine. |
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Keywords: | Chemodosimetric probe Hydrazine Rhodamine &ldquo Turn-on&rdquo fluorescence Cell imaging |
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