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Response surface methodology toward the optimization of high-energy carotenoid extraction from Aristeus antennatus shrimp
Authors:Thalia Tsiaka  Panagiotis Zoumpoulakis  Vassilia J. Sinanoglou  Constantinos Makris  Georgios A. Heropoulos  Antony C. Calokerinos
Affiliation:1. Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece;2. Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, 48, Vassileos Constantinou Avenue, 11635 Athens, Greece;3. Instrumental Food Analysis Laboratory, Department of Food Technology, Technological Educational Institution of Athens, Agiou Spyridonos, 12210 Egaleo, Greece
Abstract:High-energy assisted extraction techniques, like ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), are widely applied over the last years for the recovery of bioactive compounds such as carotenoids, antioxidants and phenols from foods, animals and herbal natural sources. Especially for the case of xanthophylls, the main carotenoid group of crustaceans, they can be extracted in a rapid and quantitative way with the use of UAE and MAE.
Keywords:UAE, ultrasound-assisted extraction   MAE, microwave-assisted extraction   RSM, response surface methodology   US, ultrasound   MW, microwaves   BBD, Box&ndash  Behnken design   ANOVA, analysis of variance   LoD, limit of detection   LoQ, limit of quantification
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