Synthesis of magnetic framework composites for the discrimination of Escherichia coli at the strain level |
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Authors: | Ji-Ping Wei Bin Qiao Wen-Jun Song Tao Chen Fei li Bo-Zhi Li Jin Wang Ye Han Yan-Feng Huang Zhi-Jiang Zhou |
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Affiliation: | 1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;2. Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China;3. Tianjin Source Environment Technology & Engineering Co., Ltd., Tianjin 300190, China;4. State Key Laboratory of Hollow Fiber Membrane Material and Membrane Process, College of Environmental and Chemical Engineering Sciences, Tianjin Polytechnic University, 399 West Binshui Road, Tianjin 300387, China;5. State Key Laboratory of Medicinal Chemical Biology (Nankai University), Nankai University, 94 Weijin Road, Tianjin 300071, China |
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Abstract: | Rapid and efficient characterization and identification of pathogens at the strain level is of key importance for epidemiologic investigations, which still remains a challenge. In this work, solvothermically Fe3O4-COOH@MIL-101 composites were fabricated by in situ crystallization approach. The composites combine the excellent properties of both chromium (III) terephthalate (MIL-101) and carboxylic-functionalized magnetite (Fe3O4-COOH) particles and possess the efficient peptides/proteins enrichment properties and magnetic responsiveness. Fe3O4-COOH@MIL-101 composites as magnetic solid phase extraction materials were used to increase the discriminatory power of MALDI-TOF MS profiles. BSA tryptic peptides at a low concentration of 0.25 fmol μL−1 could be detected by MALDI-TOF MS. In addition, Fe3O4-COOH@MIL-101 composites were successfully applied in the selective enrichment of the protein biomarkers from bacterial cell lysates and discrimination of Escherichia coli at the strain level. This work provides the possibility for wide applications of magnetic MOFs to discriminate pathogens below the species level. |
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Keywords: | Enrichment Biomarker Magnetic framework composites MALDI-TOF MS Escherichia coli |
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