Polyelectrolyte-based electrochemiluminescence enhancement for Ru(bpy)3 loaded by SiO2 nanoparticle carrier and its high sensitive immunoassay |
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Authors: | Zhi-Li Ge Tian-Mei Song Zhe Chen Wu-Run Guo Hong-Ping Xie Lian Xie |
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Affiliation: | 1. College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China;2. College of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, PR China |
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Abstract: | In this paper the strong electrochemiluminescence (ECL) nanoparticles have been prepared based on the anionic polyelectrolyte sodium polyacrylate (PAA)-ECL enhancement for Ru(bpy)32+, which were loaded by the carrier of SiO2 nanoparticle. There were two kinds of Ru(bpy)32+ for the as-prepared nanoparticles, the doped one and the exchanged one. The former was loaded inside the ECL nanoparticles by doping, in a form of ion-pair macromolecules PAA–Ru(bpy)32+. The corresponding ECL was enhanced about 2 times owing to the doping increase of Ru(bpy)32+. The latter was loaded on the PAA-doped Nafion membrane by ion exchange. The corresponding ECL was enhanced about 3 times owing to the ion-exchanging increase of Ru(bpy)32+. At the same time, ECL intensity of the doped-inside Ru(bpy)32+ was further enhanced 13 times because polyelectrolyte PAA in the doped membrane could obviously enhance electron transfer between the doped Ru(bpy)32+ and the working electrode. Furthermore, based on hydrophobic regions of the doped membrane antibody labeling could be easily realized by the as-prepared nanoparticles and then a high sensitive ECL immunoassay for HBsAg was developed. The linear range was between 1.0 and 100 pg mL−1 (R2 = 0.9912). The detection limit could be as low as 0.11 pg mL−1 (signal-to-noise ratio = 3). |
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Keywords: | Electrochemiluminescence enhancement Ion-pair macromolecule doping Polyelectrolyte-doped membrane Strong electrochemiluminescence nanoparticles High sensitive immunoassay |
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