Feasibility of application of conductometric biosensor based on acetylcholinesterase for the inhibitory analysis of toxic compounds of different nature |
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| Authors: | KV Stepurska ОО Soldatkin IS Kucherenko VM Arkhypova SV Dzyadevych AP Soldatkin |
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| Institution: | 1. Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150, Zabolotny Str., Kyiv 03680, Ukraine;2. Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64, Volodymyrska Str., Kyiv 01003, Ukraine;3. UMR 5280, Institut des Sciences Analytiques, Université de Lyon, Villeurbanne, France |
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| Abstract: | This study was aimed at the development of a conductometric biosensor based on acetylcholinesterase considering the feasibility of its application for the inhibitory analysis of various toxicants. In this paper, the optimum conditions for enzyme immobilization on the transducer surface are selected as well as the optimum concentration of substrate for inhibitory analysis. Sensitivity of the developed biosensor to different classes of toxic compounds (organophosphorus pesticides, heavy metal ions, surfactants, aflatoxin, glycoalkaloids) was tested. It is shown that the developed biosensor can be successfully used for the analysis of pesticides and mycotoxins, as well as for determination of total toxicity of the samples. A new method of biosensor analysis of toxic substances of different classes in complex multicomponent aqueous samples is proposed. |
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| Keywords: | Toxic compounds Inhibitory analysis Conductometric biosensor Acetylcholinesterase |
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