A two-photon fluorescent probe with a large turn-on signal for imaging hydrogen sulfide in living tissues |
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| Authors: | Kaibo Zheng Weiying Lin Li Tan Dan Cheng |
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| Institution: | 1. Institute of Fluorescent Probes for Biological Imaging, University of Jinan, Jinan, Shandong 250022, PR China;2. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China |
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| Abstract: | A two-photon fluorescence turn-on H2S probe GCTPOC–H2S based on a two-photon platform with a large cross-section, GCTPOC, and a sensitive H2S recognition site, dinitrophenyl ether was constructed. The probe GCTPOC–H2S exhibits desirable properties such as high sensitivity, high selectivity, functioning well at physiological pH and low cytotoxicity. In particular, the probe shows a 120-fold enhancement in the presence of Na2S (500 μM), which is larger than the reported two-photon fluorescent H2S probes. The large fluorescence enhancement of the two-photon probe GCTPOC–H2S renders it attractive for imaging H2S in living tissues with deep tissue penetration. Significantly, we have demonstrated that the probe GCTPOC–H2S is suitable for fluorescence imaging of H2S in living tissues with deep penetration by using two-photon microscopy. The further application of the two-photon probe for the investigation of biological functions and pathological roles of H2S in living systems is under progress. |
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| Keywords: | Two-photon fluorescent probe H2S Bioimaging Tissues |
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