A fluorescent polymer dots positive readout fluorescent quenching lateral flow sensor for ractopamine rapid detection |
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| Authors: | Cong Ying Shi Ning Deng Jia Jie Liang Ke Nan ZhouQiang Qiang Fu Yong Tang |
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| Affiliation: | Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China |
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| Abstract: | A fluorescent polymer dots positive readout and sensitive lateral flow assay (LFA) based on fluorescent quenching has been developed to detect ractopamine (Rac), a chemical residue in food, harmful to human health. Compared with traditional LFA strips, these fluorescent quenching LFA (FQLFA) strips provide a positive correlation method that allows users to obtain results from a weak fluorescent signal. The immunoassay strip scheme is based on the fact that fluorescent polymer dots (FPDs) in close proximity to gold nanoparticles (AuNPs) represent a strong fluorescent quenching. We show that the FQLFA strips can be used as a source to quantitatively analyze Rac in phosphate buffers (PB), swine urine and muscle tissue samples. The lowest detection limitation of the FQLFA was 0.16 ng mL−1. Our results indicated that this novel scheme was more suitable for rapid detection of small molecules. |
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| Keywords: | FRET, fluorescence resonance energy transfer LFA, lateral flow assay Rac, ractopamine Rac-HCl, ractopamine hydrochloride McAbs, monoclonal antibody FQLFA, fluorescent quenching LFA FPDs, fluorescent polymer dots AuNPs, gold nanoparticles PB, phosphate buffer NC, nitrocellulose Ab-AuNPs, antibody-labeled AuNPs LDL, lowest detection limit |
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