Anisotropy resolved multidimensional emission spectroscopy (ARMES): A new tool for protein analysis |
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| Authors: | Radu Constantin Groza Boyan LiAlan G Ryder |
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| Institution: | Nanoscale Biophotonics Laboratory, School of Chemistry, National University of Ireland, Galway, Galway, Ireland |
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| Abstract: | Structural analysis of proteins using the emission of intrinsic fluorophores is complicated by spectral overlap. Anisotropy resolved multidimensional emission spectroscopy (ARMES) overcame the overlap problem by the use of anisotropy, with chemometric analysis, to better resolve emission from different fluorophores. Total synchronous fluorescence scan (TSFS) provided information about all the fluorophores that contributed to emission while anisotropy provided information about the environment of each fluorophore. Here the utility of ARMES was demonstrated via study of the chemical and thermal denaturation of human serum albumin (HSA). |
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| Keywords: | Fluorescence Anisotropy Multidimensional Protein Unfolding Chemometrics |
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