Tubing modifications for countercurrent chromatography (CCC): Stationary phase retention and separation efficiency |
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Authors: | Michael Englert Walter Vetter |
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Institution: | University of Hohenheim, Institute of Food Chemistry, Garbenstrasse 28, D-70599 Stuttgart, Germany |
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Abstract: | Countercurrent chromatography (CCC) is a separation technique in which two immiscible liquid phases are used for the preparative purification of synthetic and natural products. In CCC the number of repetitive mixing and de-mixing processes, the retention of the stationary phase and the mass transfer between the liquid phases are significant parameters that influence the resolution and separation efficiency. Limited mass transfer is the main reason for peak broadening and a low number of theoretical plates along with impaired peak resolution in CCC. Hence, technical improvements with regard to column design and tubing modifications is an important aspect to enhance mixing and mass transfer. |
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Keywords: | Countercurrent chromatography Instrumentation Separation efficiency Tubing modification Column Multilayer coil |
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