3-D microarray and its microfabrication-free fluidic immunoassay device |
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Authors: | Yingshuai Liu Yuanyuan Zhang Zhisong Lu Chang Ming Li |
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Institution: | 1. Institute for Clean Energy & Advanced Materials, Faculty of Materials & Energy, Southwest University, Chongqing 400715, China;2. Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing 400715, China;3. Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Southwest University, Chongqing 400715, China |
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Abstract: | Conventional 2-D microarray is known to have high-throughput detection capability; however, the sensing spots density is significantly hindered by the spot-to-spot distance (gap) requirement for eliminating cross-talks between adjacent spots. Herein a new conceptual 3-D microarray device is proposed to significantly improve the spots density. To demonstrate advantages of the 3-D array, a microfabrication-free fluidic immunoassay device is further made by simply coupling an antibodies-arrayed glass cuboid into a circular glass tube. Rapid, sensitive and high-throughput flow-through immunoassays were accomplished with the 3-D array-based device for detection limits of 10–100 pg mL−1 and wide dynamic range over 4–5 orders of magnitude in human serum with cancer biomarkers α-fetoprotein (AFP) and carcinoembryonic antigen (CEA) as model targets, holding great promise for practical clinical applications. The 3-D microarray device not only significantly increases the density of sensing spots, but also greatly enhances the mass transport for rapid immunoassay when using in a flow-through device. |
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Keywords: | 3-Dimensional microarray High-throughput Microfabrication-free Flow-through immunoassay Cancer biomarkers |
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