Application of photocatalytic cadmium sulfide nanoparticles to detection of enzymatic activities of glucose oxidase and glutathione reductase using oxidation of 3,3′,5,5′-tetramethylbenzidine |
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Authors: | Ruta Grinyte Gaizka Garai-IbabeLaura Saa Valeri Pavlov |
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Affiliation: | Biofunctional Nanomaterials Unit, CIC BiomaGUNE, Parque Tecnológico de San Sebastian, Paseo Miramón 182, Donostia-San Sebastián 20009, Spain |
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Abstract: | It was found out that semiconductor CdS nanoparticles (NPs) are able to catalyze photooxidation of the well known chromogenic enzymatic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) by oxygen. The photocatalytical oxidation of TMB does not require hydrogen peroxide and its rate is directly proportional to the quantity of CdS NPs produced in situ through the interaction of Cd2+ and S2− ions in an aqueous medium. This phenomenon was applied to development of colorimetric sensitive assays for glucose oxidase and glutathione reductase based on enzymatic generation of CdS NPs acting as light-powered catalysts. Sensitivity of the developed chromogenic assays was of the same order of magnitude or even better than that of relevant fluorogenic assays. The present approach opens the possibility for the design of simple and sensitive colorimetric assays for a number of enzymes using inexpensive and available TMB as a universal chromogenic compound. |
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Keywords: | Biosensing Quantum dots Glucose Enzyme catalysis |
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