Preparation and Characterization of CdHgTe Nanoparticles and Their Application on the Determination of Proteins |
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Authors: | Fang Mei Xi-Wen He Wen-You Li Yu-Kui Zhang |
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Institution: | (1) Department of Chemistry, Nankai University, Tianjin, 300071, People’s Republic of China;(2) National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, 116011, People’s Republic of China |
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Abstract: | CdHgTe nanoparticles (NPs) with the emission in the near-infrared regions were prepared in aqueous solution, and were characterized
by transmission electron microscopy, X-ray diffraction spectrometry, spectrofluorometry and ultraviolet-visible spectrometry.
Based on the fluorescence quenching of CdHgTe NPs in the presence of proteins, a novel method for the determination of proteins
with CdHgTe NPs as a near-infrared fluorescence probe was developed. Maximum fluorescence quenching was observed with the
excitation and emission wavelengths of 500 and 693 nm, respectively. Under the optimal conditions, the calibration graphs
were linear in the range of 0.04 × 10−6–5.6 × 10−6 g ml−1 for lysozyme (Lyz) and 0.06 × 10−6–6.1 × 10−6 g ml−1 for bovine hemoglobin (BHb), respectively. The limits of detection were 13 ng ml−1 for Lyz and 27 ng ml−1 for BHb, respectively. Four synthetic samples were determined and the results were satisfied. |
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Keywords: | CdHgTe nanoparticles Near-infrared Fluorescence probe Protein Determination |
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