Salt effects in anion exchange displacement chromatography: Comparison of pentosan polysulfate and dextran sulfate displacers |
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Authors: | S. D. Gadam S. M. Cramer |
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Affiliation: | (1) Isermann Department of Chemical Engineering, Rensselaer Polytechnic Institute, 12180 Troy, NY, USA |
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Abstract: | Summary Experimental studies were carried out to investigate the utility of pentosan polysulfate as a low molecular weight polyelectrolyte displacer for the purification of proteins in anion-exchange displacement systems. In addition, the influence of mobile phase salt concentration on displacer efficacy, protein-protein resolution, and displacement development were studied for several anionic displacers. It was found that while large polyelectrolytes (50 kd dextran sulfate) were efficient displacers for a wide range of salt concentrations, relatively small polyelectrolytes (3 kd pentosan polysulfate) were seen to act as an efficient displacer only under conditions of high salt micro-environments. In addition, for proteins exhibiting similar affinities, zone mixing at the protein-protein boundary was found to be quite sensitive to the salt concentration. Finally, displacement chromatography was successfully implemented for the separation of proteins from milk whey. |
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Keywords: | Column liquid chromatography Ion-exchange chromatography Protein purification Pentosan polysulfate displacers Dextran sulfate displacers |
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