| 用结晶紫测定DNA 的分光光度法 |
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| 引用本文： | 方光荣,宋功武,李瑛.用结晶紫测定DNA 的分光光度法[J].分析测试学报,2000,19(2):44-47. |
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| 作者姓名： | 方光荣 宋功武 李瑛 |
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| 作者单位： | 湖北大学分析测试中心，湖北武汉 430062 |
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| 基金项目： | 中国分析测试基金，99-11， |
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| 摘 要： | 研究了结晶紫与ＤＮＡ的可见吸收光谱和提出了测定ＤＮＡ的分光光度法。在ｐＨ９．５６的条件下，加入ＤＮＡ后结晶紫在５８９ｎｍ的最大吸收峰强度显著下降，下降程度与ＤＮＡ的含量呈线性关系，确定了实验的最佳条件，ＤＮＡ线性响应范围为０～５ｍｇ／Ｌ，检出限１９．５μｇ／Ｌ。该法简便，快速，具有较高的选择性，对合成样品中ＤＮＡ的测定结果令人满意。
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| 关 键 词： | 结晶紫 DNA 吸收光谱法 测定方法 |
| 文章编号： | 1004－4957（2000）02－0045－02 |
| 修稿时间： | 1999年6月21日 |
Determination fo DNA by spectrophotomety with crystal violet |
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| FAng Guang rong,Song gong wu,Li ying.Determination fo DNA by spectrophotomety with crystal violet[J].Journal of Instrumental Analysis,2000,19(2):44-47. |
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| Authors: | FAng Guang rong Song gong wu Li ying |
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| Abstract: | An analytical method fo rthe determination of DNA was established by spectrophotometry ,based on the decrease of absorbance at maximum absorption wavelength for crystal violet (589nm)with addition of calf thymus DNA The degree of decrease of the absorbance is linear with the amount of DNA The determination conditions were optimized The detection limit of 19.5ug/L adn linear range of 0~5mg/L for the method were found The method is simple ,rapid and selective ,and selective ,and was applied to analysis synthetic sampe with satisfactory |
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| Keywords: | Crystal violet DNA Absorption spectrometry |
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