Hybrid reflecting objectives for functional multiphoton microscopy in turbid media |
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Authors: | Vucini? Dejan Bartol Thomas M Sejnowski Terrence J |
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Institution: | Howard Hughes Medical Institute, Computational Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA. dejan@salk.edu |
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Abstract: | Most multiphoton imaging of biological specimens is performed using microscope objectives optimized for high image quality under wide-field illumination. We present a class of objectives designed de novo without regard for these traditional constraints, driven exclusively by the needs of fast multiphoton imaging in turbid media: the delivery of femtosecond pulses without dispersion and the efficient collection of fluorescence. We model the performance of one such design optimized for a typical brain-imaging setup and show that it can greatly outperform objectives commonly used for this task. |
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