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表面增强拉曼光谱技术检测新型冠状病毒刺突蛋白
引用本文:黄景林, 周民杰, 乐玮, 等. 表面增强拉曼光谱技术检测新型冠状病毒刺突蛋白[J]. 强激光与粒子束, 2020, 32: 069001. doi: 10.11884/HPLPB202032.200145
作者姓名:黄景林  周民杰  乐玮  陈果  倪爽  牛高  李赜宇  赵宗清  何智兵  李波  赵松楠  张保汉  杜凯
作者单位:中国工程物理研究院 激光聚变研究中心,四川 绵阳 621900
摘    要:

开发具有高灵敏度、高准确性的新型冠状病毒(SARS-CoV-2)快速检测技术对疫情防控具有重要作用。本文利用表面增强拉曼光谱(SERS)技术对人体唾液中的痕量SARS-CoV-2病毒刺突蛋白(S蛋白)进行了检测。结果表明,含S蛋白的唾液样本与原始唾液样本的拉曼光谱具有显著区别,含S蛋白的唾液样本谱图中可清晰观察到属于S蛋白的拉曼谱线。该结果为后续SERS技术在SARS-CoV-2病毒快速检测方面的应用奠定了坚实基础。



关 键 词:表面增强拉曼光谱   新型冠状病毒   刺突蛋白   快速检测
收稿时间:2020-05-20
修稿时间:2020-05-30

Detection of spike protein of SARS-CoV-2 by surface enhanced Raman spectroscopy
Huang Jinglin, Zhou Minjie, Le Wei, et al. Detection of spike protein of SARS-CoV-2 by surface enhanced Raman spectroscopy[J]. High Power Laser and Particle Beams, 2020, 32: 069001. doi: 10.11884/HPLPB202032.200145
Authors:Huang Jinglin  Zhou Minjie  Le Wei  Chen Guo  Ni Shuang  Niu Gao  Li Zeyu  Zhao Zongqing  He Zhibing  Li Bo  Zhao Songnan  Zhang Baohan  Du Kai
Affiliation:Research Center of Laser Fusion, CAEP, P. O. Box 919-988, Mianyang 621900, China
Abstract:The development of rapid detection technique with high sensitivity and high veracity for SARS-CoV-2 is of great importance for the prevention and control of epidemic. In this work, the trace spike protein (S protein) of SARS-CoV-2 in human saliva was detected by surface enhanced Raman spectroscopy (SERS) technique. Significant differences can be found between the Raman spectra of the S protein stained saliva sample and the primitive saliva sample. These results will lay the foundation for the future rapid detection of SARS-CoV-2 by SERS technique.
Keywords:surface enhanced Raman spectroscopy  SARS-CoV-2  spike protein  rapid detection
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