| 一阶导数光谱法测定阿维菌素B1的含量 |
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| 引用本文： | 阿木古楞,吴晓薇,孟根达来. 一阶导数光谱法测定阿维菌素B1的含量[J]. 光谱学与光谱分析, 2001, 21(1): 84-86 |
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| 作者姓名： | 阿木古楞 吴晓薇 孟根达来 |
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| 作者单位： | 1. 内蒙古农业大学基础课部,
2. 内蒙古医学院附属医院药局, |
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| 摘 要： | 用UV－2401 PC紫外分光光度计及光谱工作站（日本岛津），以1nm狭缝宽度，2nm△λ间隔，在190－400nm波长范围内快速扫描采集阿维菌素B1紫外吸收光谱，拟合一阶导数光谱发现峰顶A252.2，和峰谷A256.8在波长坐标上的位置不受不同制剂干扰。因此认为△A=A252.2-A256.8作阿维菌素B1含量测量的定量分析依据，可以减少误差。通过对系列浓度标准品溶液一阶导数光谱分析，获得标准回归方程c=272.7△A-0.161，相关系数r=0.9996。进一步测定了不同剂制剂中阿维菌素B1的含量和其加样回收率，并获得理想结果。
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| 关 键 词： | 阿维菌素B1 一阶导数光谱法 定量分析 外吸收光谱 抗寄生虫药物 |
| 修稿时间： | 1999-09-06 |
Determination of Avermectin B1 Content by First-order Derivative Spectrum Analysis |
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| Guleng AMU,Xiaowei WU,Gendalai MENG. Determination of Avermectin B1 Content by First-order Derivative Spectrum Analysis[J]. Spectroscopy and Spectral Analysis, 2001, 21(1): 84-86 |
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| Authors: | Guleng AMU Xiaowei WU Gendalai MENG |
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| Affiliation: | Inner Mongolia University of Agriculture, 010018 Huhhot. |
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| Abstract: | After acquiring the ultraviolet absorption spectrum and its first order derivative spectrum distributions of Avermectin B1 by using UV-2401 PC spectrophotometer and spectrum-measuring system, we found that coordinates of the peak A252.2 and the valley A256.8 are not disturbed by the different forms of preparations. We considered that delta A = A252.2-A256.8 is the reliable ground for quantitative analysis of Avermectin B1 content in order to reduce error. The standard regression equation c = 272.7 delta A-0.161 and the related coefficient r = 0.9996 are obtained through measurements of the standard preparations. Then the Avermectin B1 content and the average recovery rate of different preparations are determined and the result indicates that this method is especially suitable for determining the Avermectin B1 content. |
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| Keywords: | Avermectin B 1 First-order derivative spectrum analysis Ultraviolet absorption spectrum |
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