| CULTURE OF WHEAT PROTOPLAST ——HIGH FREQUENCY MICROCOLONY FORMATION AND PLANT REGENERATION |
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| 作者姓名： | 王海波 李向辉 孙勇如 陈炬 朱祯 方仁 王培 魏建昆 |
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| 作者单位： | Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Hebei Academy of Agricultural Sciences，Shijiazhuang，PRC，Hebei Academy of Agricultural Sciences，Shijiazhuang，PRC |
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| 基金项目： | This work was supported in part by the Monsando Inc., USA. |
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| 摘 要： | Embryogenic calli were induced from the mature seeds of the hexaploid semi-winter wheat. The embryogenic cell line was established, and then the friable calli suitable for suspension were induced by adjusting the content of reduced N and changing the 2,4DAA concentration. Protoplasts were isolated from the suspension cells and cultured(?)in KM8P and some other media. A great number of microcolonies were obtained. By altering media, the microcolonies were promoted to grow further and form compact or nodule-like calli. Intact plants were regenerated through organogenesis and embryogenesis on differentiation media.
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