Determination of 1-Hydroxy Pyrene in Fish Bile by Synchronous Fluorescence Spectrometry

Environmental pollution with polynuclear aromatic hydrocarbons (PAHs) has being concerned. PAHs are mainly bound to sediment particles in the aquatic environment, but may still be biologically available to marine organisms^1]. So PAHs could be harmful to human beings by food chain transportation and accumulation. Routine monitoring of PAHs levels in the aquatic environment usually involves the determination of parent PAHs in sediment samples. Additional information on the actual uptake of these compounds by aquatic organisms can be obtained by a biomonitoring approach. However, the biotransformation rates of PAHs in fish are relatively high, and the analysis of parent PAHs in liver and muscle tissue is difficulty. Therefore, the biomonitoring of PAHs uptake should be concerned on the determination of PAHs metabolites in excreta. In this study, a simple, fast and easy operation synchronous fluorimetry has been established for this purpose.