Direct Electrochemical Study of the Redox Species in RC Protein and its Mutants Immobilized in Self-Assembly Monolayers |
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| Authors: | CAO Yi-Bin CHEN Dan-Dan WU Xing-Liang KONG Ji-Lie ZOU Yong-Long XU Chun-He |
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| Institution: | 1. Department of Chemistry, Fudan University, Shanghai 200433;
2. Shanghai institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032 |
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| Abstract: | 4-Aminothiophenol (4-ATP) self-assembled monolayer (SAM) was immobilized on gold electrode. The multi-layered protein film electrode was prepared to rinse the 4-ATP-Au electrode in poly-styrenesulfonate (PSS) and poly-dimerthyldiallylammonium chloride (PDDA) successively, then soaked in a solution containing photosynthetic reaction center (RC) protein from Rhodobacter sphaeriodes or its pigment-replaced mutant. Thus, RC was found embedded in an ordered-orientation film. In cyclic voltammetry (CV) and square wave voltammetry (SWV), Au electrode gave a series of electrochemical signals due to the redox reaction of RC protein or its mutants. Intermolecular direct electron transfer (ET) was studied in this work. |
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