Aluminum Sol-Gel Immobilized Amperometric Biosensor for H2O2

The determination of hydrogen peroxide is of practical importance in chemical, biological, clinical and many other fields. Enzyme electrodes have been studied widely for the past two decades and many research papers have been devoted to them. A very important factor in enzyme electrode development is the enzyme immobilization. Since Braun et al reported the first attempts to encapsulate proteins inside silica glasses in 1990, the low temperature sol-gel process has become an attractive avenue for the immobilization of biomolecules in connection with the development of new biosensors. A sufficient amount of trapped interstitial water contained in gels played an important role in the retention of the tertiary structure and reactivity of the immobilized enzymes.