Study on the Nitroxide-linked Naphthalene as a Probe for Hydroxyl Radicals

Reactive oxygen species (ROS),such as superoxide radical (O₂^-)and hydroxyl radical (OH), are thought to be involved in the action of many toxins and several human diseases. Among the various radicals,the hydroxyl radical is presumed to play a central role due to its strong activity. Several methods have been developed to detect hydroxyl radicals. Among them, one of the most commonly used is ESR method. Because of a high-cost instrument, this method is not suitable for routine analysis. Another method is aromatic hydroxylation. Though this method is highly sensitive, the multiple hydroxylation products make the quantitative detection of hydroxyl radical complex.