Effect of enzymatic interesterification on melting point of palm olein |
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Authors: | Yassin Atif A A Mohamed Ibrahim O Ibrahim Mohd N Yusoff Mohd S A |
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Affiliation: | (1) Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang Selangor, Malaysia;(2) Malaysian Palm Oil Board, Bandar Baru Bangi, 43000 Kajang Selangor, Malaysia |
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Abstract: | Immobilized PS-C ‘Amano’ II lipase was used to catalyze the interesterification of palm olein (POo) with 30, 50, and 70% stearic acid in n-hexane at 60°C. The catalytic performance of the immobilized lipase was evaluated by determining the composition change of fatty acyl groups and triacylglycerol (TAG) by gas liquid chromatography and high-performance liquid chromatography, respectively. The interesterification process resulted in the formation of new TAGs, mainly tripalmitin and dipalmitostearin, both of which were absent in the original oil. These changes in TAG composition resulted in an increase in slip melting point, from the original 25.5°C to 36.3, 37.0, and 40.0°C in the modified POo with 30, 50, and 70% stearic acid, respectively. All the reactions attained steady state in about 6 h. This type of work will find great applications in food industries, such as confectionery. |
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Keywords: | Interesterification PS-C ‘ Amano’ II lipase palm olein stearic acid slip melting point |
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