| ESTABLISHMENT OF T-DNA-CONTAINING SOYBEAN CELL LINES |
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| 作者姓名： | 蒋兴邨 邵启全 周泽其 王连铮 尹光初 雷勃钧 |
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| 作者单位： | Institute of Genetics，Academia Sinica，Beijing，Institute of Genetics，Academia Sinica，Beijing，Institute of Genetics，Academia Sinica，Beijing，Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin，Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin，Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin |
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| 摘 要： | This paper reports establishment of stable diploid cell lines containing nopaline synthase genes from callus containing T-DNA after 65 generations of successive suspension cultures. The strain C_(58) Agrobacterium tumefaciens was used for tumor inducing on variety "Siyuchuang". The T-DNA-containing tissue was separated in auxin-free MS medium by strictly screening three times from single cells. The presence of nopaline was identified by paper electrophoresis. The T-DNA-containing polyploid soybean cell lines were obtained at the same time. The nopaline synthase genes from T-DNA were stably integrated and expressed in soybean genome, which can be used in gene engineering with soybean.
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