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Precision of Raman depolarization and optical attenuation measurements of sound tooth enamel
Authors:Michael G Sowa  Dan P Popescu  Jeffrey Werner  Mark Hewko  Alex C-T Ko  Jeri Payette  Cecilia C S Dong  Blaine Cleghorn  Lin-P’ing Choo-Smith
Institution:(1) Institute for Biodiagnostics, National Research Council Canada, 435 Ellice Ave., Winnipeg, Manitoba, R3B 1Y6, Canada;(2) Department of Restorative Dentistry, Faculty of Dentistry, University of Manitoba, 780 Bannatyne Ave., Winnipeg, Manitoba, R3E 0W2, Canada;(3) Department of Dental Clinic Sciences, Faculty of Dentistry, Dalhousie University, 5981 University Ave., Halifax, Nova Scotia, B3H 3J5, Canada
Abstract:The demineralization of enamel that is associated with early caries formation affects the optical properties of the enamel. Polarized Raman spectroscopy and optical coherence tomography have been used to detect these changes and potentially offer a means to detect and monitor early caries development. The total optical attenuation coefficient as measured by optical coherence tomography and the polarization anisotropy of the Raman peak arising from the symmetric ν1 vibration of $${\text{PO}}^{{{\text{3 - }}}}_{{\text{4}}} $$ at approximately 959 cm−1 have been demonstrated as being sensitive markers of early caries. This ex vivo study on extracted human teeth demonstrates that these measurements can be made with reasonable precision with concomitantly good repeatability and reproducibility in sound enamel. Such reliability is crucial for these techniques to have a practical clinical value.
Keywords:Repeatability  Reproducibility  Variance components  Dental caries  Optical coherence tomography  Raman spectroscopy
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