| 毕赤酵母密码子用法对人骨形成蛋白6表达的影响分析 |
| |
| 引用本文： | 乔玉欢,米东,杨爽,朱天慧.毕赤酵母密码子用法对人骨形成蛋白6表达的影响分析[J].实验室科学,2010,13(5):82-85. |
| |
| 作者姓名： | 乔玉欢 米东 杨爽 朱天慧 |
| |
| 作者单位： | 南开大学医学院,天津300071 |
| |
| 摘 要： | 对已筛选出的毕赤酵母阳性转化子菌株（His＋Mut＋）和（His＋Muts）进行PCR验证,用SDS-PAGE和Western blot方法检测重组骨形成蛋白6的表达情况,为了解释无表达的结果,对酵母的密码子与重组骨形成蛋白6的密码子兼容性进行了理论上的初步分析。
|
| 关 键 词： | 毕赤酵母 重组骨形成蛋白6 密码子兼容性 |
Effect of codon usage on expression of recombinant human bone morphogenetic protein-6 in Pichia Pastoris |
| |
| QIAO Yu-huan,MI Dong,YANG Shuang,ZHU Tian-hui.Effect of codon usage on expression of recombinant human bone morphogenetic protein-6 in Pichia Pastoris[J].Laboratory Science,2010,13(5):82-85. |
| |
| Authors: | QIAO Yu-huan MI Dong YANG Shuang ZHU Tian-hui |
| |
| Institution: | (School of Medicine,Nankai University,Tianjin 300071,China) |
| |
| Abstract: | The screened strains （His＋Mut＋）and（His＋Muts）haboring multi-copy of human bone morphogenetic protein-6 expression cassette is confirmed by PCR.SDS-PAGE silver staining and western blot are applied to detect expression of recombinant human bone morphogenetic protein-6.Since there is no indication of expression,a theoretical analysis is performed to study the compatibility of gene code between Pichia Pastoris and human bone morphogenetic protein-6.The results show that the synonymous codon of glutamic acid and arginine of Pichia Pastoris is different from that in human bone morphogenetic protein-6. |
| |
| Keywords: | Pichia Pastoris recombinant human bone morphogenetic protein-6 compatibility of codon |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
