Detection and Characterization of Membrane Microheterogeneity by Resonance Energy Transfer |
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| Authors: | Luís M. S. Loura Rodrigo F. M. de Almeida Manuel Prieto |
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| Affiliation: | (1) Centro de Química-Física Molecular, Instituto Superior Técnico, P-1049-001 Lisboa, Portugal;(2) Departamento de Química, Universidade de Évora, Rua Romão Ramalho, 59, P-7000-671 Évora, Portugal |
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| Abstract: | The application of resonance energy transfer (RET) in the study of heterogeneity in membrane systems is described. Useful formalisms for monophasic and biphasic systems are presented, together with quantitative studies. Evidence for reduction of dimensionality, probe segregation, and microdomain sizes in these systems is discussed. Selected examples of multicomponent systems (natural membranes or model systems including proteins) are also referred, as well as recent work using RET under the microscope. |
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| Keywords: | Energy transfer lipid domain membranes phase separation |
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