| 应用光亲和分子进行PEG的光照修饰及蛋白质的光照偶联 |
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| 引用本文： | 尉继征,杨立新,胡秀丽,黄宇彬,陈学思,景遐斌. 应用光亲和分子进行PEG的光照修饰及蛋白质的光照偶联[J]. 高分子学报, 2011, 0(11): 1341-1348 |
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| 作者姓名： | 尉继征 杨立新 胡秀丽 黄宇彬 陈学思 景遐斌 |
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| 作者单位： | 1. 中国科学院长春应用化学研究所高分子物理与化学国家重点实验室 长春130022;中国科学院研究生院 北京100049
2. 中国科学院长春应用化学研究所高分子物理与化学国家重点实验室 长春130022 |
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| 基金项目： | 中国科学院百人计划(项目号KGCX2-YW-802); 国家重点基础研究发展计划(973计划,项目号2009CB930102); 国家高技术研究发展计划(863计划,项目号2007AA03Z535); 国家自然科学基金青年科学基金(基金号51003101) |
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| 摘 要： | 合成了一种光活性标记分子-对叠氮苯甲酸,将其偶联到具有双羟基的碳酸酯与乳酸的共聚物P (LA-co-DHP)上,获得了具有光反应活性的可生物降解共聚物P(LA-co-DAP),在光照条件下,可以将蛋白质方便快捷地共价偶联到P(LA-co-DHP)聚合物纤维上.在溶液中进行PEG与对叠氮苯甲酸的光照反应,通过核磁共振光谱...
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| 关 键 词： | 光亲和标记 光敏感聚合物 蛋白质固定 PEG修饰 |
PEG MODIFICATION AND PROTEIN CONJUGATION BY VIRTUE OF 4-AZIDOBENZOIC ACID AS A PHOTOAFFINITY MOLECULE |
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| WEI Jizheng , YANG Lixin , HU Xiuli , HUANG Yubin , CHEN Xuesi , JING Xiabin. PEG MODIFICATION AND PROTEIN CONJUGATION BY VIRTUE OF 4-AZIDOBENZOIC ACID AS A PHOTOAFFINITY MOLECULE[J]. Acta Polymerica Sinica, 2011, 0(11): 1341-1348 |
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| Authors: | WEI Jizheng YANG Lixin HU Xiuli HUANG Yubin CHEN Xuesi JING Xiabin |
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| Affiliation: | WEI Jizheng1,2,YANG Lixin1,HU Xiuli1,HUANG Yubin1,CHEN Xuesi1,JING Xiabin1(1State Key Laboratory of Polymer Physics and Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022)(2Graduate School of the Chinese Academy of Sciences,Beijing 100049) |
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| Abstract: | A photoaffinity labeling molecule,4-azidobenzoic acid,was synthesized and introduced into P(LA-co-DHP) to form a photoactive copolymer P(LA-co-DAP).Tests showed that proteins could be immobilized on the polymer fibers of P(LA-co-DAP) within 5 min through a photoaffinity reaction.This suggested potential applications of photoaffinity coupling in protein immobilization and protein detection.Furthermore,PEG and 4-azidobenzoic acid were reacted in solution under UV irradiation to achieve the modification on PEG... |
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| Keywords: | Photoaffinity labeling Photoactive polymer Protein immobilization PEG modification |
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