An adeno-associated viral vector transduces the rat hypothalamus and amygdala more efficient than a lentiviral vector |
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| Authors: | Marijke WA de Backer Carlos P Fitzsimons Maike AD Brans Mieneke CM Luijendijk Keith M Garner Erno Vreugdenhil and Roger AH Adan |
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| Institution: | (1) Rudolf Magnus Institute of Neuroscience, Department of Neuroscience and Pharmacology, University Medical Centre Utrecht, Utrecht, the Netherlands;(2) Medical Pharmacology Department, Leiden/Amsterdam Center for Drug Research, Leiden University Medical Center, Leiden University, Leiden, the Netherlands |
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| Abstract: | Background This study compared the transduction efficiencies of an adeno-associated viral (AAV) vector, which was pseudotyped with an
AAV1 capsid and encoded the green fluorescent protein (GFP), with a lentiviral (LV) vector, which was pseudotyped with a VSV-G
envelop and encoded the discosoma red fluorescent protein (dsRed), to investigate which viral vector transduced the lateral
hypothalamus or the amygdala more efficiently. The LV-dsRed and AAV1-GFP vector were mixed and injected into the lateral hypothalamus
or into the amygdala of adult rats. The titers that were injected were 1 × 108 or 1 × 109 genomic copies of AAV1-GFP and 1 × 105 transducing units of LV-dsRed. |
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