Comparative study of some commercial chemically bonded phases in classical reversed-phase chromatography and in ion-pair reversed-phase liquid chromatography |
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Authors: | C. Gonnet C. Bory G. Lachatre |
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Affiliation: | (1) Laboratoire de Chimie Analytique III Université Lyon I, 43 boulevard du 11 Novembre 1918, F-69622 Villeurbanne Cedex, France;(2) Laboratoire de Biochimie Hopital Debrousse, 29 rue Soeur Bouvier, F-69322 Lyon Cedex I, France;(3) Service de Pharmacologie clinique, C.H.U. Dupuytren, F-87000 Limoges, France |
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Abstract: | Summary The behaviour of various octadecyl commercial bonded phases are compared in classical reversed-phase chromatography and in ion-pair reversed-phase chromatography. Great differences are exhibited by the packings studied according to the polarity of the solutes. Whereas hydrocarbonaceous bonded phases show very similar selectivity versus apolar or weakly polar solutes, great differences are observed when analyzing more polar solutes even when ion-pair reversed-phase chromatography is performed.Presented at the 14th International Symposium on Chromatography London, September, 1982 |
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Keywords: | Reversed-phase packings Reversed-phase chromatography Ion-pair liquid chromatography Solute polarity |
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