Optically sectioned fluorescence lifetime imaging using a Nipkow disk microscope and a tunable ultrafast continuum excitation source |
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| Authors: | Grant D M Elson D S Schimpf D Dunsby C Requejo-Isidro J Auksorius E Munro I Neil M A A French P M W Nye E Stamp G Courtney P |
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| Affiliation: | Physics Department, Imperial College London, Prince Consort Road, London SW7 2BW, UK. |
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| Abstract: | We demonstrate an optically sectioned fluorescence lifetime imaging microscope with a wide-field detector, using a convenient, continuously tunable (435-1150 nm) ultrafast source for fluorescence imaging applications that is derived from a visible supercontinuum generated in a microstructured fiber. |
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